Proton pumping in cytochrome c oxidase: The coupling between proton and electron gating

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Proton pumping in cytochrome c oxidase: the coupling between proton and electron gating.

C ytochrome c oxidase is the terminal oxidase in cellular respiration. This membrane protein accepts electrons from ferrocytochrome c in the periplasmic space of the mitochondrion, one electron at a time, and transfers the reducing equivalents to the binuclear heme-iron copper site (the socalled Fea3, CuB site), where dioxygen binds and the O·O bond is subsequently cleaved (1). In this manner, ...

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Proton-pumping mechanism of cytochrome C oxidase.

Cytochrome c oxidase (CcO), as the terminal oxidase of cellular respiration, coupled with a proton-pumping process, reduces molecular oxygen (O(2)) to water. This intriguing and highly organized chemical process represents one of the most critical aspects of cellular respiration. It employs transition metals (Fe and Cu) at the O(2) reduction site and has been considered one of the most challeng...

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Kinetic gating of the proton pump in cytochrome c oxidase.

Cytochrome c oxidase (CcO), the terminal enzyme of the respiratory chain, reduces oxygen to water and uses the released energy to pump protons across a membrane. Here, we use kinetic master equations to explore the energetic and kinetic control of proton pumping in CcO. We construct models consistent with thermodynamic principles, the structure of CcO, experimentally known proton affinities, an...

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The mechanism of proton pumping by cytochrome c oxidase

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ژورنال

عنوان ژورنال: Proceedings of the National Academy of Sciences

سال: 2010

ISSN: 0027-8424,1091-6490

DOI: 10.1073/pnas.1004050107